| Comparative Study
of Several Lymphocyte Functions in Two Strains of
Mice with Different Models of Endotoxic Shock
V.
M. VÍCTOR, D. RUBIO, M. DE LA FUENTE
Department of Animal Physiology, Faculty of
Biological Sciences, Complutense University,
Madrid, Spain
Received July 30, 2001
Accepted September 26, 2001
Summary
Previously, the changes in phagocyte
functions such as adherence, chemotaxis or TNFa
production were found to be associated with
oxidative stress in endotoxin-induced septic
shock. However, in this type of oxidative stress
the lymphocyte involvement has rarely been
studied. In the present report, we analyzed the
above functions in peritoneal lymphocytes from
male and female BALB/c mice with a lethal
endotoxic shock caused by intraperitoneal
injection of E. coli lipopolysaccharide (LPS)
(100 mg/kg), male and female Swiss mice with
lethal endotoxic shock caused by intraperitoneal
injection of LPS (150 and 250 mg/kg,
respectively) or non-lethal endotoxic shock (100
mg/kg). In peritoneal lymphocytes obtained at 0,
2, 4, 12 or 24 h after LPS injection, the first
two functions of these cells in the immune
response, i.e. adherence to tissues and directed
migration (chemotaxis), were studied. At 0, 0.5,
1, 1.5, 2, 4, 12 and 24 h after LPS injection,
TNFa released by lymphocytes was also analyzed.
The results show that endotoxic shock increases
the adherence and TNFa release, and decreases the
chemotaxis of peritoneal lymphocytes. These
changes were more significant in mice with lethal
than with non-lethal endotoxic shock, a fact that
confirms the important role of lymphocytes during
endotoxic shock.
Key
words
Lymphocyte
functions · Endotoxic shock · Mice
Reprint
requests
Dr. Mónica De la Fuente, Departamento de
Biología Animal II (Fisiología Animal),
Facultad de Ciencias Biológicas, Universidad
Complutense, Jose Antonio Novais s/n, 28040
Madrid, Spain. E-mail: mondelaf@bio.ucm.es
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