Effect of Nitric
Oxide Donors on Isoprenaline-Induced Lipolysis in
Rat Epididymal Adipose Tissue: Studies in
Isolated Adipose Tissues and Immobilized Perfused
Adipocytes
D.
LINCOVÁ, D. MIŠEKOVÁ, E. KMONÍČKOVÁ, N.
CANOVÁ, H. FARGHALI
Institute of Pharmacology, First Faculty of
Medicine, Charles University, Prague, Czech
Republic
Received October 10, 2001
Accepted January 10, 2002
Summary
The present investigation was directed
to study the effect of in vitro or ex vivo NO
donors, sodium nitroprusside and molsidomine,
using isolated sliced adipose tissue or in the
form of immobilized and perfused adipocytes on
the basal and isoprenaline-stimulated lipolysis.
The results demonstrated that 1) in vitro
application of sodium nitroprusside to perfused
adipocytes or molsidomine to sliced adipose
tissues affects isoprenaline-induced lipolysis in
two ways, an increase in lipolysis at low
isoprenaline concentrations (which means the
sensitization of adipose tissues to adrenergic
effect by NO) and decreased adrenergic
agonist-stimulated lipolysis at higher
concentration of isoprenaline (a decrease in the
maximum lipolytic effect of isoprenaline), 2) low
concentrations of molsidomine alone induced
lipolysis from adipose tissue which attained more
than 60 % of that by isoprenaline (pD2 value for
molsidomine = 11.2, while pD2 for isoprenaline =
8.17) while sodium nitroprusside did not affect
the basal lipolysis significantly, 3) in vivo
administration of molsidomine for 2 days reduced
the maximum lipolytic effect of isoprenaline and
(only non-significantly) increased the
sensitivity to low doses of isoprenaline. In
conclusion the present data demonstrate that NO
plays an important role in adrenergic lipolysis
in adipose tissues and further investigations are
needed to unravel the exact role of NO in
lipolysis. .
Key
words
Adipose
tissue · Lipid
mobilization · Nitric oxide · Isoprenaline · Molsidomine · Sodium nitroprusside
Reprint
requests
Doc. MUDr. Dagmar Lincová, CSc., Institute
of Pharmacology, First Medical Faculty, Charles
University, 128 00 Prague 2, Albertov 4, Czech
Republic. E-mail: dlin@lf1.cuni.cz
|