Lidocaine-Containing Euro-Colins Solution
Prevents Renal Injury in the Isolated Perfused Canine Kidney
Exposed to Prolonged Cold Ischemia
N. ERKASAP, E. ATES1,
S. ERKASAP1, Z. KAYGISIZ
Department of Physiology and 1Department
of General Surgery, Osmangazi University, Medical Faculty,
Eskisehir, Turkey
Received October, 19,
2001
Accepted January 21, 2002
Summary
Previous studies have reported a decreased incidence of delayed
graft function after cadaveric transplantation with the use of
lidocaine pretreatment of the donor. We evaluated the effects of
lidocaine on prolonged cold ischemia and reperfusion injury in a
canine model of isolated kidney perfusion (IPK). The purpose of
this study was to evaluate the renal function of isolated
perfused canine kidneys after 48 h of cold storage with
Euro-Collins (EC) solution or EC solution plus lidocaine.
Isolated perfused canine kidneys were randomized into four
groups which contained six kidneys: I) cold flush with EC
solution and immediately reperfused, II) cold flush with EC
solution plus lidocaine and immediately reperfused, III) 48 h of
cold storage with EC and reperfusion, IV) 48 h of cold storage
with EC solution plus lidocaine and reperfusion. The measured
renal functions were glomerular filtration rate, urine
production, perfusate flow, urinary lactic dehydrogenase (ULDH),
Na reabsorptive capacity, and tissue MDA levels. Histological
examination was performed after reperfusion. The tubular
functions of kidneys preserved with EC solution containing
lidocaine were better when compared with the kidneys preserved
with EC alone. Tubular injury marker levels (ULDH) in group IV
were significantly lower than in group III and lidocaine also
reduced lipid peroxidation during reperfusion. This is in
agreement with the histological results. The results of the
present study can be taken as evidence of the cytoprotective
effect of lidocaine, which may therefore be accepted as a useful
agent for kidney preservation.
Key
words
Cold-storage · Lidocaine · Kidney · Isolated kidney perfusion
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requests
Dr. Serdar Erkasap, Hasan Polatkan Bulvari, Akin Sitesi, No:
122/13, Eskisehir, Turkey, fax : +90 222 2393772, e-mail:
serkasap@ogu.edu.tr
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