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Effect of Liposomal Muramyl Tripeptide
Phosphatidyl-ethanolamine and Indomethacin on Hematopoietic
Recovery in Irradiated Mice
N. O. MACKOVÁ, P. FEDOROČKO
Department of Cellular and Molecular Biology,
Faculty of Science, P. J. Šafárik University Košice, Slovak
Republic
Received December 17,
2001
Accepted February 22, 2002
Summary
The effects of liposomal muramyl tripeptide
phosphatidylethanolamine (MTP-PE/MLV, radioprotective
immunomodulator; 10 mg/kg) and indomethacin (INDO, inhibitor of
prostaglandin production; 2 mg/kg) on post-irradiation recovery
of hematopoietic functions in mice were investigated. Two agents
with distinct radioprotective mechanisms were administered alone
or in combination 24 h and 3 h before exposure to 7 Gy 60Co
radiation. In the post-irradiation period (3-14 days) combined
pre-treatment of mice accelerated recovery of bone marrow
cellularity, weight of spleen and myelopoietic and
erythropoietic activity in both hematopoietic organs, compared
to treatment with MTP-PE/MLV or indomethacin alone. In the
peripheral blood, improved radioprotective effects of combined
drug administration were found in the recovery of reticulocytes
and platelet count. No further significant differences in the
recovery of leukocyte count were observed in the examined groups
until post-irradiation day 14. Within the first 3-6
post-irradiation days, the bone marrow and peripheral blood
smears of mice pre-treated with indomethacin alone or its
combination with MTP-PE/MLV more frequently featured blast cells
and large cells with abundant cytoplasm which could be
considered the hematopoietic stem cells.
Key
words
Radioprotection · Hematopoiesis · Muramyl peptide · Indomethacin
Reprint
requests
Dr. Nadežda O. Macková, Department of Cellular and Molecular
Biology, Faculty of Science, P.J. Šafárik University, Moyzesova
11, 041 67 Košice, Slovakia, fax: 00421 55 6222124, e-mail:
nmackova@kosice.upjs.sk
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