|
Escherichia coli Administered into Pig Amniotic
Cavity Appear in Fetal Airways and Attract Macrophages into
Fetal Lungs
I. ŠPLÍCHAL, I.
TREBICHAVSKÝ, A. ŠPLÍCHALOVÁ1, L. DÍTĚTOVÁ, M.
ZAHRADNÍČKOVÁ
Division of Immunology and Gnotobiology,
Institute of Microbiology, Academy of Sciences of the Czech
Republic, Nový Hrádek, 1PRO.MED.CS and Institute of
Experimental Biopharmacy,
Academy of Sciences of the Czech Republic,
Hradec Králové, Czech Republic
Received November 11,
2001
Accepted February 13, 2002
Summary
Escherichia coli (2x104 bacteria) of the
non-pathogenic O86 strain or enteropathogenic O55 strain were
administered into the pig amniotic cavity at 79 to 86 days of
gestation for six or ten hours. Translocation of bacteria into
fetal lungs was confirmed by cultivation as well as by light and
electron microscopy. Infection caused an influx of macrophages
that were immunostained in cryostat sections by monoclonal
antibody recognizing calprotectin.
Key
words
Intraamniotic infection · Escherichia coli · Pig · Fetal lungs ·
Macrophages
Reprint
requests
Igor Šplíchal, Institute of Microbiology AS CR, 549 22 Nový
Hrádek, Fax +420 441-478264,
e-mail
splichal@biomed.cas.cz
|
