The Effect of Hyperbaric Oxygen Treatment on Oxidative
Stress in Experimental Acute Necrotizing Pancreatitis
Department of Emergency Medicine, Division of Surgery,
1Department of Underwater Medicine, 2Department
of Internal Medicine, 3Department of Physiology,
4Department of Toxicology, 5Department
of Surgery, Gülhane Military Medical Academy, Gülhane
School of Medicine, Ankara, Turkey
M. YASAR, S. YILDIZ1, R. MAS2, K.
DUNDAR1, A. YILDIRIM, A. KORKMAZ3, C.
AKAY4, N. KAYMAKCIOGLU5, T. OZISIK, D.
Received February 2, 2002
Accepted May 6, 2002
Various protocols may be used for acute pancreatitis treatment.
Recently, the benefit of hyperbaric oxygen (HBO) has been
demonstrated. To clarify the mechanism of HBO on the process of
the acute pancreatitis, we determined the levels of antioxidant
enzymes in an acute pancreatitis model. Forty-five Sprague-Dawley
rats were randomly divided into three groups: Group I: sham
group (n=15), Group II: pancreatitis group (n=15), Group III:
pancreatitis group undergoing HBO therapy (n=15). HBO was
applied postoperatively for 5 days, two sessions per day at 2.5
fold absolute atmospheric pressure (ATA) for 90 min. Superoxide
dismutase (Cu/Zn-SOD), malondialdehyde (MDA), and glutathione
peroxidase (GSH Px) activity were measured in pancreatic tissue
and erythrocyte lysate. MDA and GSH Px were also determined in
plasma. In addition, amylase levels were measured in the serum.
While serum amylase levels and MDA values in erythrocyte, plasma
and pancreatic tissue were decreased, the levels of GSH Px and
SOD were found to be significantly increased in the Group III as
compared to those of the Group II. The findings of our study
suggest that HBO has beneficial effects on the course of acute
pancreatitis and this effect may occur through the antioxidant
Acute pancreatitis • Hyperbaric oxygen • Antioxidant enzymes
Mehmet Yasar, MD., Gulhane Askeri Tıp Fak, GATA Loj. Serter Apt.
No. 15, 06018 Etlik, Ankara, Turkey, fax: +90 312 352 81 81,