SHORT COMMUNICATION
Oxidative Stress in the Brain Tissue of
Laboratory Mice with Acute Post Insulin Hypoglycemia
J. PATOČKOVÁ1,
P. MARHOL2, E. TŮMOVÁ1, M. KRŠIAK1,
R. ROKYTA4,
S. ŠTÍPEK5, J. CRKOVSKÁ5, M. ANDĚL3
1Department of Pharmacology, 2Department
of Chemistry and Toxicology and Cell Biochemistry, 3Second
Department of Internal Medicine, 4Department of
Normal, Pathological and Clinical Physiology, Third
Faculty of Medicine, and 5First Department of
Chemistry and Biochemistry, First Faculty of Medicine,
Charles University, Prague, Czech Republic
Received May 30, 2001
Accepted July 7, 2002
Summary
Malondialdehyde (MDA), Cu,Zn-superoxide dismutase (Cu,Zn-SOD)
and selenium-dependent glutathione peroxidase (GSPHx) are
currently considered to be basic markers of oxidative stress.
MDA is one of the end-products of the peroxidation of membrane
lipids, whereas enzymes Cu,Zn-SOD and GSHPx belong to the
natural antioxidants. The role of oxygen free radicals in the
pathogenesis of many diseases is well documented. The aim of
this study was to ascertain the influence of insulin-induced
acute hypoglycemia on oxidative stress in the brain tissue.
Hypoglycemia was induced in ICR mice by intraperitoneal
administration of insulin at a dose 24 IU/kg. There was a
correlation between the severity of hypoglycemia and the levels
of MDA, Cu,Zn-SOD and GSHPx. The results showed that in severe
hypoglycemia (serum glucose concentration below 1.0 mmol/l) the
lipoperoxidation in brain tissue expressed as the level of MDA
was higher in comparison with normoglycemic controls (glycemia
around 3.7 mmol/l) as well as in comparison with the levels of
MDA during moderate hypoglycemia (glycemia ranging between 1-2
mmol/l). This indicates the enhancement of lipoperoxidation in
the brain tissue during severe hypoglycemia. However, both
enzymes – Cu,Zn-SOD or GSHPx – did not show a similar tendency.
Key
words
Malondialdehyde • Cu,Zn-superoxide dismutase •
Selenium-dependent glutathione peroxidase • Insulin-induced
hypoglycemia
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Patočková Jitka
M.D., Department of Pharmacology, Charles University, Third
Faculty of Medicine, Ruská 87, 100 00 Praha 10, Czech republic,
Fax +420 267 102 461, E-mail:
Jitka.Patockova@lf3.cuni.cz
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