Physiol. Res. 52: 95-100, 2003

Different Patterns of Serum Interleukin 10 Response to Treatment with Anti-Tumor Necrosis Factor α Antibody (Infliximab) in Crohn´s Disease


1Department of Internal Medicine, Dérer´s Hospital, Medical Faculty of Comenius University,
2Institute of Preventive and Clinical Medicine, and 3Institute of Chemistry, Biochemistry and Clinical Biochemistry, Medical Faculty of Comenius University, Bratislava, Slovak Republic

Received February 26, 2002
Accepted August 29, 2002

Administration of anti-tumor necrosis factor antibody (anti-TNF, infliximab) down-regulates T helper 1 (Th 1) cytokines production in intestinal mucosa of patients with Crohn´s disease (CD). Interleukin 10 (IL-10) is thought to be involved in CD pathogenesis through regulation of the Th 1 response. The aim of this study was to determine the IL-10 response in CD patients treated with anti-TNF. Fourteen patients with active CD received 5 mg/kg of infliximab; clinical activity assessed by Crohn´s Disease Activity Index (CDAI), α1-acid glycoprotein and serum IL-10 were determined before and after treatment, in month 0, 1 and 5. In the group with a good clinical response, IL-10 levels diminished significantly in month 1 (p<0.05) and remained decreased in month 5. The group with a lower response showed a significant increase in IL-10 levels in month 1 (p<0.05). α1-acid glycoprotein levels obtained before treatment were significantly elevated in the group with a good clinical response (p<0.05) and a significant decrease in month 1 was observed in this group (p<0.05). These observations suggest that a pattern of IL-10 response might be related to the clinical response to anti-TNF treatment in CD.

Key words
Crohn´s disease • Anti-tumor necrosis factor therapy • Interleukin 10 • α1-acid glycoprotein

Reprint requests
Dr. Zuzana Detková, Department of Internal Medicine, Dérer´s Hospital, Medical Faculty of Comenius University, Limbova 5, 833 05 Bratislava, Slovak Republic, e-mail:

© 2003 by the Institute of Physiology, Czech Academy of Sciences