UV-B Induced Production of MMP-2 and MMP-9 in Human
Corneal Cells
I. KOZÁK, D. KLISENBAUER, T. JUHÁS
Department of Ophthalmology, P.J. Šafárik University in
Košice, Faculty of Medicine, Slovak Republic
Received January 31, 2002
Accepted June 13, 2002
Summary
The purpose of this study was to determine the production of
metalloproteinases (MMP) 2 and 9 following UV-B irradiation in
human corneal epithelial cells and fibroblasts. Epithelial cells
and fibroblasts were separated from human donor corneas and
exposed to UV-B lamp irradiation for 20, 40, 80 and 120 s. Media
samples were collected at 8, 24, 48 and 72 h and gelatinase A
and B production was assayed by the ELISA test. Statistical
significance of production was assessed by the paired t-test.
Increased production of MMP-2 was found in human corneal
fibroblasts in response to UV-B irradiation. A statistically
significant production of MMP-2 was not observed in human
corneal epithelial cells following UV-B exposure. We did not
detect any increase in MMP-9 after irradiation in either
epithelial cells or fibroblasts. MMP-2 is produced by the
corneal fibroblasts in the acute phase after UV-B irradiation.
MMP-9 is not released in vitro following UV-B irradiation damage
and therefore does not directly participate in the
pathophysiology of acute photokeratitis.
Key
words
UV-B irradiation • Human corneal epithelial cells • Human
corneal fibroblasts • MMP-2 production • MMP-9 production •
Acute photokeratitis
Reprint
requests
I. Kozák, M.D., Department of Ophthalmology, P.J. Šafárik
University in Košice, Faculty of Medicine, Trieda SNP 1, 04066
Košice, Slovak Republic. E-mail:
ikozak_2000@yahoo.com
|