RAPID COMMUNICATION
Components of Cigarette Smoke Inhibit Expansion of Oocyte-Cumulus
Complexes from Porcine Follicles
S. VRŠANSKÁ, E. NAGYOVÁ1,
A. MLYNARČÍKOVÁ, M. FICKOVÁ, J. KOLENA
Institute of Experimental Endocrinology, Slovak Academy of
Sciences, Bratislava, Slovak Republic and 1Institute
of Animal Physiology and Genetics, Academy of Sciences of
the Czech Republic, Liběchov, Czech Republic
Received November 29, 2002
Accepted February 7, 2003
Summary
The role of alkaloids in cigarette smoke was investigated in the
cumulus expansion of oocyte-cumulus complexes (OCC) isolated
from large antral porcine follicles. Suppression of the cumulus
expansion stimulated by FSH was observed in the presence of
different concentration of cadmium, anabasine and nicotine but
not its metabolite cotinine. There were comparable inhibitory
effects of cadmium and nicotine on the synthesis and
accumulation of hyaluronic acid in the cell/matrix compartment
of OCC. The inhibitory effect of tested compounds on the cumulus
expansion was accompanied by decreased progesterone synthesis by
cumulus cells during 42 h incubation of OCC with FSH. The
results suggest that cigarette smoking may affect
intrafollicular processes, which are responsible for normal
ovulation and fertilization.
Key
words
Porcine ovary • Cigarette alkaloids • Cumulus expansion •
Hyaluronic acid • Progesterone
Reprint
requests
Jaroslav Kolena, Institute of
Experimental Endocrinology, Slovak Academy of Sciences, Vlárska
3, 833 06 Bratislava, Slovak Republic. Fax: +4212 5477 4247,
E-mail: ueenkole@savba.sk
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