Red Wine Polyphenols Induce Vasorelaxation by Increased
Nitric Oxide Bioactivity
W. ZENEBE, O. PECHÁŇOVÁ ,
R. ANDRIANTSITOHAINA1
Institute of Normal and Pathological Physiology, Slovak
Academy of Sciences, Bratislava, Slovak Republic and
1Pharmacologie et Physico-Chimie des Interactions
Cellulaires et Moléculaires, Faculté de Pharmacie,
Université Louis Pasteur de Strasbourg, URA CNRS 7034,
Illkirch, France
Received
May 16, 2002
Accepted September 23, 2002
Summary
The aim of the present study was to investigate the mechanism of
vasorelaxant responses induced by red wine polyphenolic
compounds (Provinol). Rings of rat femoral artery with or
without functional endothelium were set up in a myograph for
isometric recording and precontracted with phenylephrine (10-5
M). Provinol in cumulative doses (10-9 to 10-3 mg/ml) elicited
endothelium- and dose-dependent relaxation of the artery with
maximal relaxation of 56 % at the concentration of 10-5 mg/ml.
The relaxant responses to Provinol correlated well with the
increase of NO synthase activity in the vascular tissue after
administration of cumulative doses of Provinol (10-9 to 10-3
mg/ml). NG-nitro-L-arginine methylester (L-NAME, 3x10-4 M)
significantly attenuated the endothelium-dependent relaxation
produced by Provinol. Administration of L-arginine (3x10-5 M)
restored the relaxation inhibited by L-NAME. The relaxant
responses of Provinol were abolished in the presence of
Ca2+-entry blocker, verapamil (10-6 M). Administration of
hydrogen peroxide (H2O2) abolished acetylcholine (10-5
M)-induced relaxation of the rat femoral artery, while
administration of Provinol (10-5 mg/ml) together with H2O2
helped to maintain the acetylcholine-induced relaxation.
Provinol only partially affected the concentration-response
curve for the NO donor sodium nitroprusside-induced relaxation
in rings without endothelium. In conclusion, Provinol elicited
endothelium-dependent relaxation of rat femoral artery by the
Ca2+-induced increase of NO synthase activity and by protecting
NO from degradation.
Key
words
Red wine polyphenolic compounds • Nitric oxide • Free oxygen
radicals • Endothelium • Femoral artery
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requests
O. Pecháňová, Ph.D., Institute
of Normal and Pathological Physiology, Slovak Academy of
Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovak Republic.
E-mail:
pechan@unpf.savba.sk
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