Selectins and Monocyte Chemotactic Peptide as the Markers of Atherosclerosis Activity

M. BLÁHA, J. KREJSEK¹, V. BLÁHA², C. ANDRÝS¹, D. VOKURKOVÁ¹,
J. MALÝ, M. BLAŽEK, M. SKOŘEPOVÁ

¹Second Internal Clinic, Department of Hematology, Institute of Clinical Immunology and Allergology, ²Department of Metabolic Care and Gerontology, Faculty Hospital, Charles University, Hradec Králové, Czech Republic

Received April 8, 2003
Accepted August 6, 2003



Summary
The role of adhesive selectin molecules in the process of atherogenesis is an open question. These molecules are known as markers of atherosclerosis activity, however, only some biological mechanisms are known up to now. In this study we examined the levels of soluble forms of E-, P-selectin and monocyte chemotactic protein (MCP-1) in the process of extracorporeal cholesterol elimination by LDL-apheresis. We measured the levels of sE-, sP-selectin and MCP-1 in the plasma before and after LDL-apheresis and in the washout solution from immunoabsorption columns Lipopak. Eighty measurements were performed repeatedly in 6 patients with severe familial hypercholesterolemia (FH) on long-term LDL-apheresis treatment. Before the procedure P-selectin levels were 204±179 ng/ml, E-selectin 32.1±33.7 ng/ml, MCP-1 323.8±121 pg/l, whereas after the procedure we found P-selectin levels 131.6±34 ng/ml, E-selectin 33.1±51 ng/ml, and MCP-1 200.4±15 pg/l. Levels of P- selectin were increased in the blood of patients with FH in spite of long-term intensive extracorporeal LDL-elimination, documenting thus the activity of atherosclerosis. The levels of
P-selectin and MCP-1 decreased significantly after the hypolidemic procedure and could be used as another marker showing the effectivity of the extracorporeal LDL-cholesterol elimination (immediately after the procedure), and, after further verification, may serve as a marker for controlling the therapy efficacy.


Key words
Atherosclerosis • LDL–apheresis • Selectin • Adhesive molecules