Pancreatic Islets Isolation Using Different
Protocols with in Situ Flushing and Intraductal Collagenase Injection
A. KINASIEWICZ1,2, M. JUSZCZAK3, J. PACHECKA2,
P. FIEDOR3
1Institute of Biocybernetics and Biomedical Engineering, Polish
Academy of Sciences, 2Department of Biochemistry and Clinical
Chemistry, Faculty of Pharmacy, and 3Department of General and
Transplantation Surgery, Medical University of Warsaw, Warsaw, Poland
Received February 27, 2003
Accepted July 21, 2003
Summary
Human islet transplantation seems to be a very promising clinical
procedure for patients with type I diabetes mellitus. The aim of our study
was to investigate the influence of in situ intravascular flushing with
University of Wisconsin (UW) solution and intraductal collagenase
injection at the time of pancreas procurement on the isolated islets and
exocrine tissue injury. Our experiments indicated that in situ perfusion
with the UW solution has a beneficial effect on pancreatic islets and
intraductal distention results in an increase in the concentration of
pancreatic enzymes released into the cold preservation solution during
ischemic conditions. Cold ischemia reduced islet yield, but pancreas
perfusion with the UW solution showed better ischemic tolerance of
isolated islets during glucose static incubation. We conclude that
intravascular pancreas flushing has a crucial effect on recovery and yield
of pancreatic islets and protects against exocrine tissue injury.
Key words
Pancreatic islets • Amylase • Cold ischemia • Preservation
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