L-type and T-type Ca2+
Current in Cultured Ventricular Guinea Pig Myocytes
K. ZORN-PAULY, P. SCHAFFER, B. PELZMANN, E. BERNHART, P. LANG,
B. KOIDL
Institut für Medizinische Physik und Biophysik, Graz, Austria
Received June 19, 2003
Accepted November 19, 2003
Summary
The aim of this investigation was to study L-type and T-type
Ca2+ current (ICaL and ICaT) in short-term cultured adult guinea
pig ventricular myocytes. The isolated myocytes were suspended
in serum-supplemented medium up to 5 days. Using whole-cell
patch clamp techniques ICaL and ICaT were studied by applying
voltage protocols from different holding potentials (40 and 90
mV). After 5 days in culture the myocytes still showed their
typical rod shaped morphology but a decline in cell membrane
capacitance (26 %). The peak density of ICaT was reduced
significantly between day 0 (1.60.37 pA/pF, n=9) and day 5
(0.40.13 pA/pF, n=11), whereas peak ICaL density revealed no
significant differences during culturing. The ICaT/ICaL ratio
dropped from 0.13 at day 0 to 0.05 at day 5. Compared with day 0
ICaL the steady state inactivation curve of day 1, day 3 and day
5 myocytes was slightly shifted to more negative potentials. Our
data indicate that guinea pig ventricular L-type and T-type Ca2+
channels are differently regulated in culture.
Key words
Ventricular myocytes Short-term culture L-type Ca2+ channel
T-type Ca2+ channel
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