Markers of Oxidative Stress
in Diabetic Mothers and Their Infants During Delivery
D. RAJDL1, J. RACEK1, A.
STEINEROVÁ4, Z. NOVOTNÝ2, F. STOŽICKÝ3,
L. TREFIL1, K. SIALA5
1Institute of Clinical
Biochemistry and Hematology, 2Department of
Gynecology and Obstetrics, 3Department of Pediatrics,
University Hospital and Charles University, Faculty of Medicine
in Pilsen, 4Medica Centrum, Pilsen, Czech Republic
and 5Department of Internal Medicine, St Helier
Hospital in London, United Kingdom
Received February 23, 2004
Accepted August 6, 2004
On-line available December 9, 2004
Summary
Oxidative stress is probably a pathophysiological process
leading to disadvantageous outcomes in diabetic pregnancies. We
aimed to map a complex of potential markers of oxidative stress
in this condition. Diabetic mothers had significantly higher
concentrations of thiobarbituric acid reactive substances in the
plasma [TBARS] both before (p<0.0001) and after (p<0.001)
delivery and also their newborns showed higher values of TBARS
(p<0.0001) in comparison with the control group. Diabetic
mothers also showed lower concentrations of reduced glutathione
in erythrocytes [GSH] both before (p<0.05) and after (p<0.01)
delivery and their infants also had lower levels of GSH
(p<0.0001). We found a lower total antioxidative capacity of
plasma [AOC] before delivery (p<0.05) in the diabetic group in
comparison with the control group. Newborns of diabetic mothers
had higher plasmatic concentrations of apolipoproteine B [apo B]
(p<0.05), higher erythrocyte glutathione peroxidase [GPx]
activity (p<0.05) and lower pH (p<0.001) in the umbilical cord
blood, when compared with infants of control non-diabetic
mothers. We conclude that pregestational and gestational
diabetes mellitus represent increased oxidative stress for both
mother and her infant. TBARS in plasma are a valuable marker of
oxidative stress in this condition. Disruption of glutathione
peroxidase/glutathione pattern can be involved in
pathophysiology of enhanced oxidative stress in diabetic
pregnancies.
Key words
Diabetes mellitus • Gestational diabetes mellitus • Delivery •
Oxidative stress • Gender
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