Effect of Melatonin on Brain
Oxidative Damage Induced by Traumatic Brain Injury in Immature
Rats
D. OZDEMIR1, N. UYSAL2, S.
GONENC2, O. ACIKGOZ2, A. SONMEZ2,
A. TOPCU2, N. OZDEMIR3, M. DUMAN1,
I. SEMIN2, H. OZKAN1
1Department of Pediatrics, 2Department of
Physiology, School of Medicine, Dokuz Eylul University,
Inciralti and 3Ataturk Training and Research
Hospital, Department of Neurosurgery, Izmir, Turkey
Received September 30, 2004
Accepted January 13, 2005
On-line available February 16, 2005
Summary
Progressive compromise of antioxidant defenses and free
radical-mediated lipid peroxidation, which is one of the major
mechanisms of secondary traumatic brain injury (TBI), has also
been reported in pediatric head trauma. In the present study, we
aimed to demonstrate the effect of melatonin, which is a potent
free radical scavenger, on brain oxidative damage in 7-day-old
rat pups subjected to contusion injury. Whereas TBI
significantly increased thiobarbituric acid reactive substances
(TBARS) levels, there was no compensatory increase in the
antioxidant enzymes such as superoxide dismutase (SOD) and
glutathione peroxidase (GPx) 24 hours after TBI in 7-day-old
rats. Melatonin administered as a single dose of 5 mg/kg
prevented the increase in TBARS levels in both non-traumatized
and traumatized brain hemispheres. In conclusion, melatonin
protects against oxidative damage induced by TBI in the immature
brain.
Key words
Traumatic brain injury (TBI) • Immature rat • Lipid peroxidation
• Superoxide dismutase (SOD) • Glutathione peroxidase (GPx) •
Melatonin
|