Blood Phagocyte Activation
During Open Heart Surgery With Cardiopulmonary Bypass
M. PAVELKOVÁ1, L. KUBALA1,
M. ČÍŽ1, P. PAVLÍK2, R. WAGNER2,
J. SLAVÍK2, J. ONDRÁŠEK2, J. ČERNÝ2,
A. LOJEK1
1Institute of Biophysics and 2Center of
Cardiovascular and Transplantation Surgery, Brno,
Czech Republic
Received July 7, 2004
Accepted May 10, 2005
On-line available May 24, 2005
Summary
Open heart surgery with a cardiopulmonary bypass (CPB) is
associated with a systemic inflammatory response which
significantly contributes to adverse postoperative
complications. The purpose of this study was to characterize the
activation of blood phagocytes during open heart surgery with
CPB. Blood samples were collected during and up to 24 h after
surgery. The production of reactive oxygen species (ROS) in
whole blood, the expression of surface molecules by blood
phagocytes and complement activity in the plasma were
determined. A cDNA microarray analysis of leukocyte RNA profile
of genes was performed related to the inflammatory response.
Activation of the complement was already observed at the
beginning of CPB. This was followed by an increase in the
neutrophil number and in both spontaneous and opsonized
zymosan-activated ROS production after the onset of reperfusion.
The activation of blood phagocytes was affirmed by changes in
surface receptors involved in the adhesion and migration of
leukocytes (CD11b, CD62L and CD31). Gene arrays also confirmed
the activation of leukocytes 4 h after reperfusion. In
conclusion, open heart surgery with a cardiopulmonary bypass was
found to be associated with a rapid and pronounced activation of
blood phagocytes and complement activation which was partly
independent at the onset of CPB.
Key words
Phagocytes • Complement • Surface receptors • Reactive oxygen
species • Gene expression
|
