Adhesion of Erythrocytes to
Endothelial Cells After Acute Exercise: Differences in Red Blood
Cells from Juvenile and Adult Rats
A. TEMIZ ARTMANN1, M. AKHISAROGLU2,
Z. SERCAN3, H. RESMI4, B.M. KAYATEKIN2,
K. YORUKOGLU5, G. KIRKALI4
1University of Applied Sciences in Aachen, Department
of Cell Biophysics, Jülich, Germany and Dokuz Eylul University
School of Medicine, Departments of 2Physiology,
3Medical Biology and Genetics, 4Biochemistry,
and 5Pathology, Izmir, Turkey
Received May 16, 2005
Accepted September 1, 2005
On-line available October 17, 2005
Summary
Erythrocytes (RBC) from untrained male Wistar rats and rat
glomerular endothelial cells (EC) were used to investigate the
effects of acute exercise (speed: 20 m/min, slope: 0, duration:
1 hour) on RBC membrane protein oxidation and adhesion to
cultured EC. Experimental animals were divided into juvenile
(age 10 weeks) and adult (age 30 weeks) groups for these
studies. Immediately following exercise, juvenile rat RBC
membrane protein oxidation was significantly enhanced. Adult rat
RBC showed significantly higher basal protein oxidation than
juvenile RBC; but the level of adult rat RBC membrane protein
oxidation was unaffected by exercise. Prior to exercise, adult
rat RBC showed significantly higher adhesion to EC than RBC of
juvenile rat. There was no difference in plasma fibronectin or
fibrinogen levels following exercise. Only juvenile rat RBC
showed a significant decrease in sialic acid residue content
following exercise. These experiments show that there are
changes in RBC-EC interactions following exercise that are
influenced by animal age.
Key words
Erythrocyte • Endothelium • Adhesion • Exercise
|