Adenosine Potentiates
Stimulatory Effects on Granulocyte-Macrophage Hematopoietic
Progenitor Cells in Vitro of IL-3 and SCF, but Not Those of
G-CSF, GM-CSF and IL-11
M. HOFER, A. VACEK, M. POSPÍŠIL, L. WEITEROVÁ, J.
HOLÁ,
D. ŠTREITOVÁ, V. ZNOJIL1
Institute of Biophysics, Academy of Sciences of the Czech
Republic, and 1Medical Faculty, Masaryk University,
Brno, Czech Republic
Received August 25, 2005
Accepted November 14, 2005
On-line available December 12, 2005
Summary
The aim of the studies was to ascertain if adenosine is able to
co-operate with selected hematopoietic growth factors and
cytokines, namely with granulocyte colony-stimulating factor
(G-CSF), granulocyte-macrophage colony-stimulating factor
(GM-CSF), stem cell factor (SCF), interleukin-3 (IL-3), and
interleukin-11 (IL-11), in inducing the growth of colonies from
hematopoietic progenitor cells for granulocytes and macrophages
(GM-CFC) from normal bone marrow cells in vitro. Adenosine was
found not to produce any colonies when present in the cultures
as the only potential stimulator. All the tested cytokines and
growth factors were observed to induce the growth of distinct
numbers of GM-CFC colonies, with the exception of IL-11. When
suboptimal concentrations of the evaluated cytokines and growth
factors were tested in the cultures in which various
concentrations of adenosine were concomitantly present, mutually
potentiating effects were found in the case of IL-3 and SCF.
These results confirm the role of adenosine in regulation of
granulopoiesis and predict IL-3 and SCF as candidates for
further in vivo studies of their combined administration with
adenosine.
Key words
Hematopoiesis • Adenosine • Cytokines • Hematopoietic growth
factors
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