Different Sensitivity of
Miniature Endplate Currents of the Rat Extensor Digitorum
Longus, Soleus and Diaphragm Muscles to a Novel
Acetylcholinesterase Inhibitor C-547
K. A. PETROV1, I. V. KOVYAZINA2,
V. V. ZOBOV1, E. A. BUKHARAEVA2,3,
E. E. NIKOLSKY2,3, F. VYSKOČIL4,5
1Kazan Institute of Organic and Physical Chemistry,
Russian Academy of Sciences, 2Kazan Institute of
Biochemistry and Biophysics, Russian Academy of Sciences, 3Kazan
State Medical University, Kazan, Russia, 4Department
of Animal Physiology and Developmental Biology, Faculty of
Sciences, Charles University and 5 Institute of
Physiology, Academy of Sciences of the Czech Republic, Prague,
Czech Republic
Received date March 13, 2006
Accepted date April 18, 2006
On-line available June 22, 2006
Summary
A novel derivative of 6-methyluracil, C-547, increased the
amplitude and prolonged the duration of miniature endplate
currents (MEPCs) which is typical for acetylcholinesterase
inhibition. In the soleus and extensor digitorum longus
significant potentiation was detected at nanomolar
concentrations. In contrast, in the diaphragm muscle, the
increase in the amplitudes of the MEPCs and the decay time
constant appeared only when the concentration of C-547 was
elevated to 1x10-7 M. Possible consequences for the exploitation
of this drug, which can selectively inhibit AChE in particular
synapses, are discussed.
Key words
Miniature endplate current • Acetylcholinesterase •
Anticholinesterase
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