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Effect of High Glucose
Concentrations on Expression of ELAM 1, VCAM-1 and ICAM-1 in
HUVEC with and without Cytokine Activation
TS. ALTANNAVCH1, K. ROUBALOVÁ2,
P. KUČERA1, M. ANDĚL1
1Second Department of Internal Medicine, Third
Faculty of Medicine, Charles University, 2National Institute of
Public Health, Prague, Czech Republic
Received June 6, 2002
Accepted March 10, 2003
Summary
Diabetes mellitus is associated with an increased prevalence of endothelial
dysfunction and development of atherosclerotic vascular diseases. We demonstrate
here that hyperglycemia results in the expression of adhesion molecules on
endothelial cells in vitro. Incubation of human umbilical vein endothelial cells
(HUVEC) in a culture medium with 11.0 mM, 16.5 mM and 22.0 mM glucose
concentrations induced the expression of intercellular adhesion molecule-1
(ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte
adhesion molecule-1 (ELAM-1). This effect was detectable after 24 h incubation
of HUVEC with a high glucose concentration. The effect of high glucose
concentration on TNF- induced expression of ELAM-1, VCAM-1 and ICAM-1 was
negligible, if at all. These results show that even a short-term exposure of
endothelial cells (ECs) to high glucose concentration leads to their activation
associated with increased expression of adhesion molecules such as ELAM-1,
VCAM-1 and ICAM-1.
Key words
Adhesion molecules • Atherosclerosis • Endothelial cells •
Diabetes mellitus • High glucose
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