Physiol. Res. 53: 77-82, 2004

Effect of High Glucose Concentrations on Expression of ELAM 1, VCAM-1 and ICAM-1 in HUVEC with and without Cytokine Activation


1Second Department of Internal Medicine, Third Faculty of Medicine, Charles University, 2National Institute of Public Health, Prague, Czech Republic

Received June 6, 2002
Accepted March 10, 2003

Diabetes mellitus is associated with an increased prevalence of endothelial dysfunction and development of atherosclerotic vascular diseases. We demonstrate here that hyperglycemia results in the expression of adhesion molecules on endothelial cells in vitro. Incubation of human umbilical vein endothelial cells (HUVEC) in a culture medium with 11.0 mM, 16.5 mM and 22.0 mM glucose concentrations induced the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1). This effect was detectable after 24 h incubation of HUVEC with a high glucose concentration. The effect of high glucose concentration on TNF- induced expression of ELAM-1, VCAM-1 and ICAM-1 was negligible, if at all. These results show that even a short-term exposure of endothelial cells (ECs) to high glucose concentration leads to their activation associated with increased expression of adhesion molecules such as ELAM-1, VCAM-1 and ICAM-1.

Key words
Adhesion molecules • Atherosclerosis • Endothelial cells • Diabetes mellitus • High glucose

© 2004 by the Institute of Physiology, Czech Academy of Sciences