Conserved mechanism of Xrn1 regulation by glycolytic flux and protein aggregation

Lecturer: Satyendra Mondal, MSc. / Department of Functional Organisation of Biomembranes

Annotation: The regulation of eukaryotic gene expression via mRNA degradation is an evolutionarily conserved process, with the 5′-3′ exoribonuclease 1 (Xrn1) playing a pivotal role. In yeast, following the depletion of fermentable carbon source, Xrn1 is sequestered at the plasma membrane-associated protein complex, the eisosome, where it becomes inactive. In this study, we show that the plasma membrane localisation of Xrn1 requires the core eisosome components and identify a putative eisosome-binding domain of Xrn1. Our findings demonstrate that the eisosome binding of Xrn1 is subject to the halting of ongoing glycolytic flux rather than the availability of fermentable carbon sources. Furthermore, we show that the localisation of the human orthologue of Xrn1 resembles that of its yeast counterpart when expressed in yeast. Our results advance our understanding of the plasma membrane-associated regulation of Xrn1 and indicate that the regulation principle may be evolutionarily conserved from yeast to humans.

The seminar will take place on 7. 11. 2024 from 2 p.m. in the Turquoise Auditorium of the IEM CAS.

We look forward to your participation.