Dear Colleagues,

the Institute of Molecular Genetics of the Czech Academy of Sciences would like to invite you to the 5-day course „Processing and analysis of microscopic images in biomedicine (PAMIB25)“ intended for PhD students and junior researchers.

The course will address fundamental aspects of image data acquisition, processing, and analysis, encompassing techniques in stereology. Alongside theoretical principles, the course will prioritize hands-on practical learning. Participants will gain proficiency in utilizing the freely available software package Fiji for both basic and advanced analyses. They will learn to assess co-localizations, analyze data from FRAP and electron microscopy, track particles, segment objects in images, and explore methods of employing artificial intelligence for image segmentation.

Additionally, participants will master techniques to enhance data quality through deconvolution using Huygens software. An interactive session featuring Imaris software will be complemented by practical exercises. Furthermore, this year introduces a session utilizing NIS-Elements, a platform for image processing developed in Prague, and a lecture about the publishing images in scientific journals. Independent practical tasks involving Fiji and Huygens will also constitute a significant component of the course.

The course will be held at the Institute of Molecular Genetics of the Czech Academy of Sciences in Prague in March 31 – April 4, 2025. For more information and link to the registration form, please, visit the course website: https://course.img.cas.cz/pamib/. The registration deadline is March 17.

While the course aligns with the Microscopy Methods in Biomedicine, prior attendance is not a prerequisite for participation.

The course will be taught in English.

On behalf of the organizers,

Michaela Blažíková and Martin Čapek