RAPID
COMMUNICATION
Gossypol
Inhibits Follicle-Stimulating Hormone- and
Epidermal Growth Factor-Stimulated Expansion of
Oocyte-Cumulus Complexes from Porcine
Preovulatory Follicles
J.
KOLENA, S. VRŠANSKÁ, E. NAGYOVÁ1,
M. JEŽOVÁ
Institute
of Experimental Endocrinology, Slovak Academy of
Sciences, Bratislava, Slovak Republic and 1Institute
of Animal Physiology and Genetics, Academy of
Sciences of the Czech Republic, Liběchov, Czech
Republic
Received
May 7, 2001
Accepted June 20, 2001
Summary
The
role of gossypol in the cumulus expansion of
oocyte-cumulus complexes (OCC) isolated from
large antral porcine follicles was investigated.
Marked suppression of cumulus expansion
stimulated with follicle-stimulating hormone
(FSH) and epidermal growth factor (EGF) was
observed in the presence of different
concentrations of gossypol. Comparable inhibitory
effects were obtained in the presence of NO
donor, S-nitroso-N-acetylpenicillamine or sodium
nitroprusside, suggesting that the inhibitory
effect of gossypol may be mediated via NO
generation. The inhibitory effect of gossypol on
cumulus expansion of OCC was accompanied by
inhibition of progesterone secretion of OCC and
the decrease of [125I]EGF binding to granulosa
cells.
Key words
Gossypol
- Cumulus expansion - Progesterone secretion-
Epidermal growth factor receptors
Reprint
requests
J.
Kolena, Institute of Experimental Endocrinology,
Slovak Academy of Sciences, Vlárska 3, 833 06
Bratislava, Slovak Republic. Fax: +421 2
54774247. E-mail: ueenkole@savba.sk
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