Volume 50, Issue 6:

RAPID COMMUNICATION


Gossypol Inhibits Follicle-Stimulating Hormone- and Epidermal Growth Factor-Stimulated Expansion of Oocyte-Cumulus Complexes from Porcine Preovulatory Follicles

J. KOLENA, S. VRŠANSKÁ, E. NAGYOVÁ1, M. JEŽOVÁ

Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic and 1Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic

Received May 7, 2001
Accepted June 20, 2001


Summary
The role of gossypol in the cumulus expansion of oocyte-cumulus complexes (OCC) isolated from large antral porcine follicles was investigated. Marked suppression of cumulus expansion stimulated with follicle-stimulating hormone (FSH) and epidermal growth factor (EGF) was observed in the presence of different concentrations of gossypol. Comparable inhibitory effects were obtained in the presence of NO donor, S-nitroso-N-acetylpenicillamine or sodium nitroprusside, suggesting that the inhibitory effect of gossypol may be mediated via NO generation. The inhibitory effect of gossypol on cumulus expansion of OCC was accompanied by inhibition of progesterone secretion of OCC and the decrease of [125I]EGF binding to granulosa cells.


Key words
Gossypol - Cumulus expansion - Progesterone secretion- Epidermal growth factor receptors

Reprint requests
J. Kolena, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlárska 3, 833 06 Bratislava, Slovak Republic. Fax: +421 2 54774247. E-mail: ueenkole@savba.sk

PHYSIOLOGICAL RESEARCH
© 2001 by the Institute of Physiology,
Czech Academy of Sciences

ISSN 0862 - 8408

Issue 6