Basic information

The course aims to teach multimodal preclinical imaging techniques, including in vivo two-photon microscopy, in vivo multi-photon-based label-free techniques (SHG, THG, CARS), and magnetic resonance imaging (MRI) of small rodents. Participants will acquire theoretical knowledge and practical skills in various neuroimaging techniques, with a focus on intravital multi-photon microscopy and MRI principles. Emphasizing the importance of animal models in physiological research, the course guides participants through the entire process of conducting preclinical bioimaging experiments. It includes a detailed pipeline for analyzing brain tumour structures and functional parameters, covering cranial window surgery, two-photon microscopy usage, perfusion MRI and subsequent image analysis. Additionally, the workshop addresses the challenges in using animal models for research and provides access to field experts.

Intended audience

The course is designed for researchers (PhD candidates, Postdocs, Principal Investigators, etc.) with a serious interest in integrating in vivo imaging into their projects.

Methods

Two-photon fluorescent microscopy (functional calcium/voltage imaging, structural imaging), second harmonic generation (SHG), third harmonic generation (THG), coherent anti-Stokes Raman spectroscopy (CARS), high field magnetic resonance perfusion imaging (MRI), fluorescence lifetime imaging microscopy (FLIM)

Registration and more information can be found on our website.