"Studying the eukaryotic cilium by a single organelle approach" Cilia are present on the surface of many eukaryotic cells and have critical motility, sensory, and signalling functions. Yet, many fundamental aspects of their biology are poorly understood. Taking advantage of the fact, that the cilia are experimentally well accessible, I have been developing approaches for… Více
Sensorineural hearing loss in mice lacking GABAB receptor-associated KCTD12 protein Lecturer: Mgr. Adolf Melichar / Department of Auditory Neuroscience Annotation: KCTD12 protein is the auxiliary subunit of the G-protein coupled receptor for γ-aminobutyric acid (GABA-B). KCTD12 constitutively binds to the intracellular portion of GABA-B receptor and significantly modulates its structural and functional properties. KCTD12/GABA-B complexes are characterized by… Více
“Canonical RNAi in vivo in mice” Canonical RNAi, i.e. long dsRNA-induced mRNA degradation, is essentially not operating in vivo in mammalian somatic cells. Low efficiency of dsRNA conversion into small interfering RNA by Dicer was identified as key limiting factor for canonical RNAi in mammals. We have genetically engineered mice to express a more active… Více
"The impact of FAM84B in retinal homeostasis" Fam84b is an incompletely characterised protein. Its aberrant expression has been associated with various human cancers, with a possible reported role in endosomal trafficking. We genetically ablated Fam84b expression in mice, and extensively characterised the subsequent age-related, degenerative phenotype in the retinas of knockout animals. The aim of… Více