Akce

“CRL4COP1 ubiquitination complex as a balancer of early odontogenesis” Cullin-RING ubiquitin ligase (CRL) complexes have been heavily studied in connection to cancer progression but there is limited evidence about their physiological roles. One of the exceptions is disruption of limb development by thalidomide induced by inhibition of CRL4 complex via regulation of Fgf8 (Ito et…
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The course is very practically-oriented, theoretical lectures are followed by 6 hours of hands-on session every day in small groups. You will learn widefield, confocal, and advanced methods including super-resolution, photomanipulation, and FLIM. You will have a chance to work on high-end microscopes and compare wide variety of approaches and instrumentation. For detailed program and…
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“Every stick has two ends – Using high resolution imaging to characterize both ends of the eukaryotic flagellum” The eukaryotic flagellum is important for cell motility. The microtubule-based axoneme generating the flagellar beating has been extensively studied. However, additional structures are associated with the proximal and distal end of the flagellum, with their composition and…
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Trace Element and Speciation Analysis in Biological and Environmental Samples

The human eye is our primary tool for interpreting images, so scientific images should be adjusted with this perception in mind. However, display and print transfer characteristics often degrade images, making them differ from reality. This course offers a unique approach, focusing on preparing image data for presentation. We’ll cover essential topics like gamma correction…
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“Quality control in snRNP biogenesis” Before being assembled into spliceosomes, small nuclear ribonucleoprotein particles (snRNPs) undergo a complex and regulated maturation pathway. Cajal bodies (CBs) have been shown to monitor the final stage of this process by sequestering immature or misassembled particles through a yet unknown quality control mechanism. Here, we have identified coilin, a…
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Lukáš Chmátal, Ph.D., Whitehead Institute for Biomedical Research, MIT, USA Heart disease is the leading cause of death worldwide, but its effects are far from uniform. Men and women experience striking differences in symptoms, prevalence, and outcomes – yet the molecular mechanisms underlying these differences remain poorly understood. In this lecture, I will reveal how…
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“Structural basis of MICAL autoinhibition and activation” In this talk, we will explore the role of MICAL proteins in cellular dynamics, focusing on their ability to disassemble actin filaments and their implications for processes like axon guidance, cytokinesis, and cell morphology. We will present our recent findings based on the cryoEM structure of human MICAL1,…
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Modeling human early pregnancy with stem cells” The blastocyst is the early mammalian organism before implantation in the uterus. We have promoted the self-organization of stem cells into models of mouse and human blastocysts, which we have named blastoids (Nature 2018, Nature 2021). Blastoids are morphologically and transcriptionally similar to the blastocyst and contain analogs…
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“Infections and hematopoietic stem cells: how do they communicate?” Infections are primarily cleared by immune cells in our bodies. However, we observed that the most immature hematopoietic stem cells also contribute to this process. In this lecture, we will report on the mechanisms that activate an hematopoietic stem cell during infection and the cellular adaptations that…
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Speaker: Martina Rauner, Technische Universität Dresden, Germany

“Tipping the Lipid Scales: Negative Feedback Regulation via ORMDLs Protein Degradation Ensures Sphingolipid Homeostasis” Sphingolipids are essential lipid molecules that contribute to cellular structure and signaling. Their de novo synthesis is initiated in the endoplasmic reticulum (ER) and catalyzed by the multimeric serine palmitoyltransferase (SPT) complex. The ER-resident ORMDL proteins are well-established regulators of sphingolipid…
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